Translational_Unit

Part:BBa_K2278022:Design

Designed by: Paul ZANONI   Group: iGEM17_INSA-UPS_France   (2017-10-08)


Lecrocin I antimicrobial peptide with Alpha-Factor Secretion Signal


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 244
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A pAOX1 promoter is present on the pPICZalpha vector before the construction. It makes genome recombination easier in Pichia pastoris genome. Part:BBa_K1800001: Alpha-Factor Secretion Signal


Source

The peptides DNA sequence has been obtained by reverse translate the amino acid sequence of the Leucrocin I proposed by Pata et al., 2011. They had determinated the amino acid sequence by mass spectrometry analysis.


References

Pata, S., Yaraksa, N., Daduang, S., Temsiripong, Y., Svasti, J., Araki, T. and Thammasirirak, S. (2011). Characterization of the novel antibacterial peptide Leucrocin from crocodile (Crocodylus siamensis) white blood cell extracts. Developmental & Comparative Immunology, 35(5), pp.545-553.